Studies of antimicrobial resistance genes using DNA probes
نویسندگان
چکیده
منابع مشابه
Studies of antimicrobial resistance genes using DNA probes.
In nature there is a theoretical gene pool from which microorganisms can acquire genetic determinants that increase their chances of survival in hostile environments. This gene pool consists of genomic DNA, plasmids, phages, insertion sequences, and transposable elements, all of which exist in a dynamic equilibrium. Among the genes that can be obtained by bacteria are those encoding antimicrobi...
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We developed and tested a glass-based microarray suitable for detecting multiple tetracycline (tet) resistance genes. Microarray probes for 17 tet genes, the beta-lactamase bla(TEM-1) gene, and a 16S ribosomal DNA gene (Escherichia coli) were generated from known controls by PCR. The resulting products (ca. 550 bp) were applied as spots onto epoxy-silane-derivatized, Teflon-masked slides by usi...
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هدف از این مطالعه بررسی امکان استفاده از داروهای شناخته شده در درمان سایر بیماریها به عنوان داروهای ضد سرطان است. همچنین با استفاده از این داروها در ساختمان کمپلکس فلز می توان شاخص های دارویی بدست آمده را بررسی نمود. داروی ضد ویروس ایدز(hiv)به نام زیدوودین(azt)انتخاب و.کمپلکس.محلول.در.آب[pt(azt)2]cl2سنتزو به روشهای مختلف فیزیکی و شیمیایی شناسایی گردید. بر هم کنش مقایسه ای این دارو و کمپلکس پلا...
15 صفحه اولDetection of antibiotic resistance genes with DNA probes.
An increasing number of reports describing the use of DNA probes for the clinical diagnosis of specific pathogens are now appearing in the scientific literature. So far as antibiotic resistance is concerned, DNA probes have been used primarily to obtain information regarding the dissemination of particular antibiotic resistance genes and to obtain information regarding their evolutionary relati...
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High throughput genotyping is essential for studying the spread of multiple antimicrobial resistance. A test oligonucleotide microarray designed to detect 94 antimicrobial resistance genes was constructed and successfully used to identify antimicrobial resistance genes in control strains. The microarray was then used to assay 51 distantly related bacteria, including Gram-negative and Gram-posit...
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ژورنال
عنوان ژورنال: Antimicrobial Agents and Chemotherapy
سال: 1986
ISSN: 0066-4804,1098-6596
DOI: 10.1128/aac.29.5.721